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Shodex HPLC Column PROTEIN LW-403 4D ID 4,6mm L 150mm - F6989403

Price: £1,925.96


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PROTEIN KW / PROTEIN LW columns separation by size also for high molecular weight

PROTEIN LW-403 4D HPLC Semi-micro column - For antibody drugs analysis - Gel Filtration (GFC) - 1.9μm - 4.6mm I.D. x 150mm Length

Shodex Product Number: F6989403

The silica-based SEC (GFC) column for proteins and enzymes

Specifications:

Description PROTEIN LW-403 4D 150x4.6mm
Chemistry Producers Term LW
Mode of Separation Gel Filtration (GFC) / SEC Size Exclusion
Functional group Hydrophilic polymer / Hydroxyl
Gel material Silica
Plates per column ≥ 11,000
Pore size 300 Å (max. 1,000 Å)
Housing material steel
Maximum pressure 20 MPa (200 bar)
Usual flow rate 0.35 mL/min
Maximum flow rate 0.5 mL/min
Temperature range 5 to 45°C
pH range 3.0 to 7.5
Salt concentration ≤ 0.3 M
Shipping solvent H2O
MW range 10,000 - 700,000
Exclusion limit (1,000,000)
Internal Diameter 4.6 mm
Length 150 mm
Brand PROTEIN
Particle Size 1.9 µm
USP L20, L33, L59

PROTEIN LW-803 and LW-403 4D Features

More and more monoclonal antibodies (mAb) are used for therapeutic drugs. The pore size of the Shodex PROTEIN LW-803 and LW-403 4D is suitable for this analysis.

  • Pore size specifically controlled for analyzing proteins with a molecular weight of several hundred of thousand
  • High performance analysis of antibody drugs and various proteins
  • High lot-to-lot reproducibility
  • Fulfills USP-NF L20 - Dihydroxypropane groups chemically bonded to porous silica or hybrid particles, 1.5-10 μm in diameter, or a monolithic silica rod.
  • Fulfills USP-NF L33 - Packing having the capacity to separate dextrans by molecular size over a range of 4,000 to 500,000 Da. It is spherical, silica-based, and processed to provide pH stability.
  • Fulfills USP-NF L59 - Packing for the size-exclusion separations of proteins (separation by molecular weight) over the range of 5 to 7000 kDa. The packing is a spherical 1.5- to 10-μm, silica or hybrid packing with a hydrophilic coating.

LW-403 4D Possible Applications

  • Comparison of SEC Separation of IgG between LW-403 4D and LW-803
  • Comparison of SEC Separation of Standard Proteins between LW-403 4D and LW-803
  • Comparison of IgG Separation with Conventional Device and Semi-micro Type Device (LW-403 4D)
  • Comparison of SEC Separation of IgG between LW-403 4D and LW-803
  • Comparison of SEC Separation of Standard Proteins between LW-403 4D and LW-803
  • Comparison of Separation of Proteins with Conventional Device and Semi-micro Type Device (LW-403 4D)
  • Durability Test by Number of Sample Injections (LW-403 4D)

Range of Aqueous SEC (GFC) Columns: Silica-based Columns

Product Code Product Name Plate Number
(TP/column)		 Particle Size
(μm)		 Pore Size
(Å)		 Column Size (mm)
I.D. x Length		 Shipping Solvent
KW-800 Series | Standard columns | Base Material: Silica 
F6989000 PROTEIN KW-802.5 ≥ 21,000 5 400 8.0 x 300 H2O
F6989103 PROTEIN KW-803 ≥ 21,000 5 1,000 8.0 x 300 H2O
F6989104 PROTEIN KW-804 ≥ 16,000 7 1,500 8.0 x 300 H2O
F6700131 PROTEIN KW-G 6B (guard column) 7 — 6.0 x 50 H2O
KW400 Series | High performance semi-micro columns | Base Material: Silica
F6989201 KW402.5-4F ≥ 35,000 3 400 4.6 x 300 H2O
F6989202 KW403-4F ≥ 35,000 3 800 4.6 x 300 H2O
F6989203 KW404-4F ≥ 25,000 5 1,500 4.6 x 300 H2O
F6989204 KW405-4F ≥ 25,000 5 2,000 4.6 x 300 H2O
F6700132 KW400G-4A (guard column) 5 — 4.6 x 10 H2O
For antibody drugs analysis | Standard columns | Base Material: Silica
F6989303 PROTEIN LW-803 ≥ 12,000 3 1,000 8.0 x 300 H2O
F6700133 PROTEIN LW-G 6B (guard column) 3 — 6.0 x 50 H2O
For antibody drugs analysis | Semi-micro columns | Base Material: Silica
F6989403 PROTEIN LW-403 4D ≥ 11,000 1.9 1,000 4.6 x 150 H2O
F6700134 PROTEIN LS-G 4J (guard column) 1.9 — 4.6 x 20 H2O

  • Model: F6989403
  • Manufactured by: Showa Denko

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