PROTEIN KW / PROTEIN LW columns separation by size also for high molecular weight
PROTEIN LW-403 4D HPLC Semi-micro column - For antibody drugs analysis - Gel Filtration (GFC) - 1.9μm - 4.6mm I.D. x 150mm Length
Shodex Product Number: F6989403
The silica-based SEC (GFC) column for proteins and enzymes
Specifications:
| Description |
PROTEIN LW-403 4D 150x4.6mm |
| Chemistry Producers Term |
LW |
| Mode of Separation |
Gel Filtration (GFC) / SEC Size Exclusion |
| Functional group |
Hydrophilic polymer / Hydroxyl |
| Gel material |
Silica |
| Plates per column |
≥ 11,000 |
| Pore size |
300 Å (max. 1,000 Å) |
| Housing material |
steel |
| Maximum pressure |
20 MPa (200 bar) |
| Usual flow rate |
0.35 mL/min |
| Maximum flow rate |
0.5 mL/min |
| Temperature range |
5 to 45°C |
| pH range |
3.0 to 7.5 |
| Salt concentration |
≤ 0.3 M |
| Shipping solvent |
H2O |
| MW range |
10,000 - 700,000 |
| Exclusion limit |
(1,000,000) |
| Internal Diameter |
4.6 mm |
| Length |
150 mm |
| Brand |
PROTEIN |
| Particle Size |
1.9 µm |
| USP |
L20, L33, L59 |
PROTEIN LW-803 and LW-403 4D Features
More and more monoclonal antibodies (mAb) are used for therapeutic drugs. The pore size of the Shodex PROTEIN LW-803 and LW-403 4D is suitable for this analysis.
- Pore size specifically controlled for analyzing proteins with a molecular weight of several hundred of thousand
- High performance analysis of antibody drugs and various proteins
- High lot-to-lot reproducibility
- Fulfills USP-NF L20 - Dihydroxypropane groups chemically bonded to porous silica or hybrid particles, 1.5-10 μm in diameter, or a monolithic silica rod.
- Fulfills USP-NF L33 - Packing having the capacity to separate dextrans by molecular size over a range of 4,000 to 500,000 Da. It is spherical, silica-based, and processed to provide pH stability.
- Fulfills USP-NF L59 - Packing for the size-exclusion separations of proteins (separation by molecular weight) over the range of 5 to 7000 kDa. The packing is a spherical 1.5- to 10-μm, silica or hybrid packing with a hydrophilic coating.
LW-403 4D Possible Applications
- Comparison of SEC Separation of IgG between LW-403 4D and LW-803
- Comparison of SEC Separation of Standard Proteins between LW-403 4D and LW-803
- Comparison of IgG Separation with Conventional Device and Semi-micro Type Device (LW-403 4D)
- Comparison of SEC Separation of IgG between LW-403 4D and LW-803
- Comparison of SEC Separation of Standard Proteins between LW-403 4D and LW-803
- Comparison of Separation of Proteins with Conventional Device and Semi-micro Type Device (LW-403 4D)
- Durability Test by Number of Sample Injections (LW-403 4D)
Range of Aqueous SEC (GFC) Columns: Silica-based Columns
| Product Code |
Product Name |
Plate Number
(TP/column) |
Particle Size
(μm) |
Pore Size
(Å) |
Column Size (mm)
I.D. x Length |
Shipping Solvent |
| KW-800 Series | Standard columns | Base Material: Silica |
| F6989000 |
PROTEIN KW-802.5 |
≥ 21,000 |
5 |
400 |
8.0 x 300 |
H2O |
| F6989103 |
PROTEIN KW-803 |
≥ 21,000 |
5 |
1,000 |
8.0 x 300 |
H2O |
| F6989104 |
PROTEIN KW-804 |
≥ 16,000 |
7 |
1,500 |
8.0 x 300 |
H2O |
| F6700131 |
PROTEIN KW-G 6B |
(guard column) |
7 |
— |
6.0 x 50 |
H2O |
| KW400 Series | High performance semi-micro columns | Base Material: Silica |
| F6989201 |
KW402.5-4F |
≥ 35,000 |
3 |
400 |
4.6 x 300 |
H2O |
| F6989202 |
KW403-4F |
≥ 35,000 |
3 |
800 |
4.6 x 300 |
H2O |
| F6989203 |
KW404-4F |
≥ 25,000 |
5 |
1,500 |
4.6 x 300 |
H2O |
| F6989204 |
KW405-4F |
≥ 25,000 |
5 |
2,000 |
4.6 x 300 |
H2O |
| F6700132 |
KW400G-4A |
(guard column) |
5 |
— |
4.6 x 10 |
H2O |
| For antibody drugs analysis | Standard columns | Base Material: Silica |
| F6989303 |
PROTEIN LW-803 |
≥ 12,000 |
3 |
1,000 |
8.0 x 300 |
H2O |
| F6700133 |
PROTEIN LW-G 6B |
(guard column) |
3 |
— |
6.0 x 50 |
H2O |
| For antibody drugs analysis | Semi-micro columns | Base Material: Silica |
| F6989403 |
PROTEIN LW-403 4D |
≥ 11,000 |
1.9 |
1,000 |
4.6 x 150 |
H2O |
| F6700134 |
PROTEIN LS-G 4J |
(guard column) |
1.9 |
— |
4.6 x 20 |
H2O |
