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DEAE Sepharose Fast Flow 500ml - 17-0709-01

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 IEX Sepharose™ Fast Flow

  • For preparative purifications, even when purifying crude samples.
  • Available with strong (Q and SP) and weak (DEAE, CM, and ANX) anion and cation exchangers, in bulk quantities and in prepacked HiTrap™ and HiPrep™ columns, as well as HiTrap™ IEX Selection Kit.
  • For separation of proteins of all pI values with Q and SP having a high dynamic capacity for proteins over the entire pH range.
  • ANX has a different selectivity compared to DEAE, and is applicable for separation of high molecular weight proteins.
  • Easy scale-up from HiTrap™ scale to laboratory scale to process scale.

IEX Sepharose™ Fast Flow

Technical Information


TECHNICAL SPECIFICATIONS
Ion exchanger type 
Q Sepharose™ FF Quaternary ammonium strong anion 
DEAE Sepharose™ FF Diethylaminoethyl weak anion 
ANX Sepharose™ 4 FF Diethylaminopropyl weak anion 
SP Sepharose™ FF Sulfopropyl strong cation 
CM Sepharose™ FF Carboxymethyl weak cation 
Ionic capacity 
Q Sepharose™ FF 0.18-0.25 mmol (Cl-)/ml 
DEAE Sepharose™ FF 0.11-0.16 mmol (Cl-)/ml 
ANX Sepharose™ 4 FF 0.13-0.18 mmol (Cl-)/ml 
SP Sepharose™ FF 0.18-0.25 mmol (H+)/ml 
CM Sepharose™ FF 0.09-0.13 mmol (H+)/ml 
Dynamic capacity 
Q Sepharose™ FF 120 mg HSA/ml medium 
DEAE Sepharose™ FF 110 mg HSA/ml medium 
ANX Sepharose™ 4 FF 5 mg thyroglobulin/ml medium 
SP Sepharose™ FF 70 mg RNase/ml medium 
CM Sepharose™ FF 50 mg RNase/ml medium 
Pressure/flow spec. 
Q Sepharose™ FF 400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm 
DEAE Sepharose™ FF 300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm 
ANX Sepharose™ 4 FF min 200 cm/h, 100 kPa, XK50/60 column, bed height 25 cm 
SP Sepharose™ FF 400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm 
CM Sepharose™ FF 300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm 
Average particle size 90 μm (45-165 μm) 
Matrix 
Sepharose™ FF Highly cross-linked agarose, 6% 
Sepharose™ 4 FF Highly cross-linked agarose, 4% 
pH stability 
Q Sepharose™ FF 1-14 (short term), 2-12 (long term) 
DEAE Sepharose™ FF 1-14 (short term), 2-13 (long term) 
ANX Sepharose™ 4 FF 2-14 (short term), 3-13 (long term) 
SP Sepharose™ FF 3-14 (short term), 4-13 (long term) 
CM Sepharose™ FF 2-14 (short term), 4-13 (long term) 
Chemical stability


 		Stable in all common aqueous buffers: 
8 M urea, 6 M guanidine HCl, 70% ethanol, 1 M NaOH*, and 1 M acetic acid*. 
Storage 20% ethanol (Q, DEAE, ANX, CM), 0.2 M sodium acetate in 20% ethanol (SP) 
Storage temperature 4°C to 30°C 
*1 M NaOH and acetic acid should be used only for cleaning purposes.

 
Recombinant human superoxide dismutase produced by Saccharomyces cerevisiae is purified directly from clarified cell homogenate on Q Sepharose™ Fast Flow after desalting and buffer exchange on Sephadex™ G-25. A 2.6 × 5 ml ion exchange column handles 390 ml of sample in 45 min.


  • Model: 17-0709-01
  • Manufactured by: GE HealthCare

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