Gel Filtration Chromatography
TSKgel SuperMultiporePW-N GFC Guard Column, 5 μm, ID 4.6 mm x Length 3.5 cm
TSKgel SuperMultiporePW columns are packed with spherical, mono-disperse, polymethacrylate particles incorporating the proprietary multi-pore particle technology. Each particle each contains a wide range of pore sizes in order to provide near linear calibration curves. They belong to the semi-micro type of SEC columns (6 mm ID, 15 cm length) providing high theoretical plate numbers at half of the length of a conventional SEC column. The TSKgel SuperMultiporePW series comprises of three column types covering different molecular weight ranges (PW-N; PW-M, PW-H).
The main application area for TSKgel SuperMultiporePW columns is the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides. Figure 1 shows the near linear calibration curves for the three SuperMultiporePW-type columns. Figure 2 shows the benefits of TSKgel SuperMultiporePW columns over a combination of different conventional SEC columns, namely the improved resolution and the smooth peak shapes without any inflection points.
|Particle size:||4 µm||5 µm||8 µm (6-10 µm)|
|Exclusion limit (Da):||120,000||1,000,000||10,000,000|
|Separation range (Da)|
|300 ~ 50,000||500,000 ~ 1,000,000||1,000~10,000,000|
|Theoretical plates:||> 16,000||> 12,000||> 7,000|
|Analysis of polyvinylpyrrolidone|
MW calibration curve
- Model: 22793
- Manufactured by: Tosoh BioScience