Waters ACQUITY UPLC Peptide (PST) BEH C18 Column, 130 Å, 1.7 µm, 2.1 mm X 50 mm, 1K - 15K, 1/pk - 186003554

Waters ACQUITY UPLC Peptide (PST) BEH C18 Column, 130Å, 1.7 µm, 2.1 mm X 50 mm, 1K - 15K, 1/pk

Product Number: 186003554

The ACQUITY BEH130 C18 Peptide Separation Technology (PST) columns have been specially optimized and QC tested for peptide separations. The small-pore (130Å) trifunctionally bonded BEH particle offers the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay samples for proteomics, protein characterization, and peptide synthesis.

PST (Peptide Separation Technology)

peptide mapping. Reversed-phase (RP) chromatography has become the separation mode of choice due to its relatively high resolving power and outstanding quantitative (UV) and qualitative (ESI-MS) information. Waters Ethylene Bridged Hybrid (BEH Technology) and new Charged Surface Hybrid (CSH™) Peptide Separation Technology columns provide capabilities that help address demanding application needs.

BEH (Ethylene Bridged Hybrid) Technology

Ethylene Bridged Hybrid (BEH) particle technology ensures maximum column performance and longer column lifetimes under all chromatographic conditions. The hybrid particle technology offers many advantages over conventional silica-based particles, including the ability to control silanol activity for better reproducibility, peak shape, and efficiency. The BEH particle is created from two high purity monomers: tetraethoxysilane (TEOS) and bis(triethoxysilyl) ethane (BTEE), which results in highly stable, pH resistant, and mechanically strong particles that set the performance standard for method development. BEH particle technology allows for seamless transfer from analytical to preparative separations.

The advantages of BEH columns include:

• Rugged hybrid organic/inorganic base particle
• Improved peak shape for bases-low silanol activity
• Wider operating pH range for method development flexibly
• Mechanical strength for maximum column efficiency at UHPLC operating pressures
• Available Particle Sizes: 1.7, 2.5, 3.5, 5, and 10 µm
• Column Chemistries: C18, C18 AX, Shield RP18, C8, Phenyl, Amide, Z-HILIC, HILIC

Specifications