PST (Peptide Separation Technology)
peptide mapping. Reversed-phase (RP) chromatography has become the separation mode of choice due to its relatively high resolving power and outstanding quantitative (UV) and qualitative (ESI-MS) information. Waters Ethylene Bridged Hybrid (BEH Technology) and new Charged Surface Hybrid (CSH™) Peptide Separation Technology columns provide capabilities that help address demanding application needs.
ACQUITY UPLC and XBridge BEH130 and BEH300 C18 Guards and Columns
- Ideally suited for the separation of wide range peptides: large and small, acidic and basic, hydrophilic and hydrophobic.
- 130Å and 300Å pore offerings for different separation selectivities and for use with polypeptides less than or greater than 10,000 Daltons.
- Scalable 1.7 µm, 3.5 µm, and 5 µm particles that address a variety of application needs.
- Specifically QC tested with tryptic digests of cytochrome c that helps ensure batch-to-batch consistency in validated methods.
BEH (Ethylene Bridged Hybrid) Technology
Ethylene Bridged Hybrid (BEH) particle technology ensures maximum column performance and longer column lifetimes under all chromatographic conditions. The hybrid particle technology offers many advantages over conventional silica-based particles, including the ability to control silanol activity for better reproducibility, peak shape, and efficiency. The BEH particle is created from two high purity monomers: tetraethoxysilane (TEOS) and bis(triethoxysilyl) ethane (BTEE), which results in highly stable, pH resistant, and mechanically strong particles that set the performance standard for method development. BEH particle technology allows for seamless transfer from analytical to preparative separations.
