Oligonucleotide Reversed-Phase Columns
Ion pairing reversed-phase (IPRP) chromatography is the method of choice for synthetic oligonucleotide characterization and impurities analysis. The method helps scientists resolve synthesis and process-related impurities better than any other chromatographic technique, including anion exchange (AEX), HILIC, and SEC. The technique is not without its challenges. Multiple factors can directly affect separation performance and reproducibility, including the purity and stability of ion-pairing reagents, non-specific adsorption to chromatographic hardware, and batch-to-batch variations in sorbent. IPRP relies on high pH and temperature conditions for optimal separations, requiring column materials to have high pH and temperature tolerance.
Gain confidence in your results using Waters Oligonucleotide Reversed Phase Columns that offer:
- Industry-leading pH and temperature stability with BEH C18 hybrid silica particle technology for exceptional resolution and column life.
- A dramatic reduction of non-specific adsorption (NSA) with MaxPeak Premier High-Performance
- Surfaces (HPS) Technology for maximum sensitivity, reproducibility, and productivity.
- Oligonucleotide-based QC testing to ensure lot-to-lot separations performance and reproducibility.
