XBridge Premier Columns
Particle Stability with MaxPeak HPS Protection
Working at the extremes of temperature and pH while maximizing analyte peak shape is a significant challenge for methods development scientists. Now, with XBridge Premier 2.5 µm and 3.5 µm Columns, it doesn’t have to be.
XBridge Premier Columns prevent undesired analyte/surface interactions through the power of MaxPeak HPS Technology, which is designed to increase analyte recovery, sensitivity, and reproducibility by minimizing analyte/surface interactions that can lead to sample losses.
XBridge Premier Columns are the evolution of Waters XBridge XP (eXtended Performance) columns and XBridge HPLC columns, and they combine the industry-leading column stability of Ethylene Bridged Hybrid (BEH) particles with MaxPeak High Performance Surfaces (HPS) Technology.
PST (Peptide Separation Technology)
peptide mapping. Reversed-phase (RP) chromatography has become the separation mode of choice due to its relatively high resolving power and outstanding quantitative (UV) and qualitative (ESI-MS) information. Waters Ethylene Bridged Hybrid (BEH Technology) and new Charged Surface Hybrid (CSH™) Peptide Separation Technology columns provide capabilities that help address demanding application needs.
- Suited for the reversed-phase analysis of hydrophobic and hydrophilic peptides at low and high pH
- Outstanding peak capacity and peak shape in TFA or FA when compared to 100% silica based C18 columns
- Two pore sizes (130 Å and 300 Å) for a wide range of peptides
- Superior peak shape and reduced tailing for acidic peptides
- Enhanced recovery and sensitivity for acidic peptides
- Available in OBD preparative 3.5 and 5 µm particles for seamless scalability and enhanced peptide isolation and purification
BEH (Ethylene Bridged Hybrid) Technology
Ethylene Bridged Hybrid (BEH) particle technology ensures maximum column performance and longer column lifetimes under all chromatographic conditions. The hybrid particle technology offers many advantages over conventional silica-based particles, including the ability to control silanol activity for better reproducibility, peak shape, and efficiency. The BEH particle is created from two high purity monomers: tetraethoxysilane (TEOS) and bis(triethoxysilyl) ethane (BTEE), which results in highly stable, pH resistant, and mechanically strong particles that set the performance standard for method development. BEH particle technology allows for seamless transfer from analytical to preparative separations.
