Waters 2475 Multi Wavelength Fluorescence Detector for Alliance HPLC Systems with Low-dispersion Analytical Flow Cell - 176017009

2475 Fluorescence (FLR) Detector

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Waters® 2475 Multi-λ Fluorescence Detector for Alliance® HPLC Systems with low-dispersion analytical flow cell

Product Number: 176017009

The culmination of HPLC sensitivity and selectivity

Waters 2475 Fluorescence (FLR) Detector offers unsurpassed sensitivity for quantifying low concentrations of target compounds, both naturally fluorescent and derivatized with a fluorescent tag. It sets the standard for fluorescence method development with 3D and “on-the-fly” spectral scanning for determining the optimal emission and excitation wavelengths and can monitor up to four independent wavelength pairs.

With the 2475 FLR Detector, your lab can achieve high sensitivity, low dispersion methods with unsurpassed accuracy, maximum uptime, and complete confidence in the analysis.

• Increase efficiency with multiple detection modes – 2D, 3D, and on-the-fly spectral scanning
• Benefit from the versatility of a low-dispersion system solution compatible with a wide range of column dimensions
• Achieve better bench-to-bench reproducibility with normalized emission units

Recommended Use: For highly sensitive quantification of very low-level components, such as environmental or food contaminants.

Reliable quantification of trace contaminants

When a highly sensitive method is required for quantification of very low-level components, such as environmental or food contaminants, fluorescence detection is the ideal detection solution due to its low limits of repeatable quantification coupled with its high selectivity for analysis in complex matrices.

For the analysis of aflatoxins, low limits of quantification are achieved with the 2475 Fluorescence Detector and post-column derivatization.

Innovation driving maximum sensitivity

The 2475 FLR Detector features a unique axially illuminated flow cell design. The excitation energy enters the rectangular flow cell along its long axis and is reflected along this axis by a mirror at the end of the flow cell. This not only minimizes unwanted scattering, but also effectively doubles the flow cell’s path length, as seen in this flow cell diagram. The resulting emission energy exits from the top of the flow cell. This revolutionary design results in less dispersion, less stray light, less volume, and more path length than a traditional cubic flow cell – ultimately delivering greater sensitivity for your fluorescence applications.

The 2475 FLR Detector’s low dispersion and high sensitivity of the flow cell allows for low detection limits with well resolved chromatographic peaks for the analysis of carbamates using post-column derivatization.

Better day-to-day and bench-to-bench reproducibility

Peak responses are available in emission units (EU), as well as energy units. Emission units are normalized to water, a universally available standard, using an automated diagnostic that eliminates peak dependence on gain settings. Factors that normally influence fluorescence measurements, such as lamp or optics degradation, can be compensated by periodically re-normalizing. Additionally, variations in fluorescence responses that occur from one 2475 FLR Detector to another are significantly reduced. Traditionally, variability is observed in the response of individual photo multiplier tubes (PMTs). Using a calibration procedure unique to the 2475 FLR Detector, repeatable PMT performance from one 2475 FLR Detector unit to another is guaranteed.

Using three-dimensional emission or excitation spectral data results in faster methods development by allowing the user to quickly determine the optimal excitation and emission wavelength settings through wavelength extraction or by using Empower Software to automatically determine the timed wavelength changes. Peak identification is enhanced through the use of both emission and excitation spectral libraries.

Intuitive software control

The 2475 FLR Detector is controlled by Waters Empower Software, offering functionality like on-the-fly spectral scanning to optimize emission, and excitation wavelengths as well as timed event programmability to achieve optimal sensitivity, based upon the auto-optimize gain utility. Empower Software offers powerful features that help you turn raw data into reports by providing streamlined data viewing, archival, and retrieval, and fully customizable reporting. Enhanced compliance-ready capabilities maintain security and comply with Federal regulatory standards such as 21 CFR Part 11.

Harness the power of 3D fluorescence detection with Empower Software. Fluorescence data can be displayed via a three-dimensional contour map giving users a “bird’s-eye-view” of the data.

Specifications:

1. All performance specifications are measured following a warm-up period of one hour with ambient Δ T ≤ ± 2.0 °C
2. U.S. Patent Numbers: 6,423,249 and 6,783,705