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Waters 2489 UV/Visible (UV/Vis) Detector

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2489 UV/Visible (UV/Vis) Detector

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The most sensitive and versatile dual-wavelength absorbance detector available

Whether yours is a laboratory performing routine UV-based applications or pushing the limits of detection with low-level impurity analyses, the Waters 2489 UV/Visible (UV/Vis) Detector is the best detection choice for performance, reliability, and usability. 

The 2489 UV/Vis Detector is the most sensitive and versatile dual-wavelength absorbance detector available for HPLC and UHPLC. It is designed to combine a high energy deuterium source, a precise optical design, and low noise, high speed electronics all in one detector. These advanced capabilities take UV/Vis detection performance to an unprecedented new level.

When operated as part of a Waters HPLC system, the 2489 UV/Vis Detector is fully controlled via Empower or MassLynx software data systems, providing intuitive data acquisition, analysis, reporting, and diagnostics tools.

When operated as part of a Waters HPLC system, the 2489 UV/Vis Detector is fully controlled via Empower or MassLynx software data systems, providing intuitive data acquisition, analysis, reporting, and diagnostics tools.

Overview

  • Identify and quantitate major and minor components in the same run with a linear range of 2.5 AU
  • Monitor absorbance at two user-selectable wavelengths, resulting in more analyte information per run, and increased productivity
  • Achieve confidence for optimal system usage with lamp serial numbers, hours, and number ignition tracking provides

Recommended Use: The best dual-wavelength absorbance detection choice for performance, reliability, and usability.

Filled with innovation - designed for usability

The 2489 UV/Vis Detector is the most sensitive and versatile dual-wavelength absorbance detector available for HPLC. The detector’s advanced features take UV/Vis detection performance to a new level, with: 

  • Enhanced sensitivity via patented TaperSlit flow cell, which channels light through the cell for better energy throughput while minimizing RI effects
  • Low noise performance (<5 µAU)
  • Lamp optimization for the best s/n performance over the lifetime of a Deuterium lamp
  • Flexible sampling rates, from 1 to 80 Hz, for normal, and fast LC separations
  • Independent optimization of sampling rates and filter time constants gives you the ability to tune your detector to your separation goals
  • Enhanced thermal wander management reduces baseline wander due to fluctuations caused by ambient conditions
  • Flow cell options to match your separation scale and type
  • Accessible detector components for easy operation and maintenance

Patented TaperSlit flow cell maximizes energy throughput while minimizing refractive index effects, maintaining good peak shape and ensuring high sensitivity and optimal performance.

Patented TaperSlit flow cell maximizes energy throughput while minimizing refractive index effects, maintaining good peak shape and ensuring high sensitivity and optimal performance.

Setting the standard for trace impurity analysis

The 2489 UV/Vis Detector offers simplicity for routine UV-based applications and high sensitivity for low-level impurity analysis. The detector’s extended linear range of 2.5 AU enables the identification and quantitation of both major and minor components in the same run - a key benefit for trace impurity or metabolism studies.

The ability to identify and quantitate low-level impurities in the same chromatographic run as the analyte of interest is a good measure of the detector’s sensitivity. The 2489 UV/Vis Detector easily integrates trace impurities down to 0.006% and lower.

The ability to identify and quantitate low-level impurities in the same chromatographic run as the analyte of interest is a good measure of the detector’s sensitivity. The 2489 UV/Vis Detector easily integrates trace impurities down to 0.006% and lower.

More analyte information per run

Dual wavelength capability is standard on the 2489 UV/Vis Detector. This gives you the flexibility to simultaneously monitor absorbance at two user-selectable wavelengths, resulting in more analyte information per run, and increased productivity. The two wavelength channels can be overlaid, ratioed, and/or summarized within the data system for better qualitative data.

Dual wavelength capability allows sensitivity and selectivity to be optimized for each analyte in a single run without the need for wavelength switching.

Dual wavelength capability allows sensitivity and selectivity to be optimized for each analyte in a single run without the need for wavelength switching.

Optimal performance across your workflows

Whether you work in research and development, analytical development, or QA/QC, the 2489 UV/Vis Detector is designed to meet your needs with a wide range of flow cell options and integrated features, while providing optimal flexibility, usability, and performance for both routine and complex samples.

As shown in this example, compounds found in natural products that exhibit weak UV absorption characteristics, such as ginsenosides found in Panax ginseng, are easily detected due to the superior sensitivity of the 2489 UV/Vis Detector.

As shown in this example, compounds found in natural products that exhibit weak UV absorption characteristics, such as ginsenosides found in Panax ginseng, are easily detected due to the superior sensitivity of the 2489 UV/Vis Detector.

Specifications:

Operating Specifications
Wavelength range 190 to 700 nm
Bandwidth ≤5 nm
Wavelength accuracy ±1 nm (via patented Erbium filter)
Wavelength repeatability ±0.1 nm
Linearity ≤5% at 2.5 AU, propylparaben, 257 nm, analytical flow cell
Baseline noise, single wavelength ≤5 x 10-6 AU peak to peak, 230 nm, 10 points/s, 1.0 s, 30 s segments, dry analytical flow cell
Baseline noise, dual wavelength ≤35 x 10-6 AU peak to peak, 230 nm, 280 nm, 1 point/s, 2.0 s, 30 s segments, dry analytical flow cell
Drift ≤1 x 10-4 AU/hour, 239 nm, 2 points/s, 1.0 s, 30 s segments, dry analytical flow cell
Measurement range 0.0001 to 4.0000 AU
Sampling rate Up to 80 points/s
Optical Component Specifications
Light source Deuterium arc lamp
Warranty: 2000 hours or 1 year (whichever comes first)
Flow cell design Patented TaperSlit
Path length 10 mm (analytical cell)
Cell volume 16.3 μL (analytical cell)
Pressure limit 1000 psi (analytical cell)
Wetted materials Alliance HPLC/ACQUITY Arc: 316 stainless-steel, fused sililca, PEEK, fluoropolymer ACQUITY Arc Bio: fluoropolymer, fused silica, MP35N, PEEK, titanium

 


  • Model: 2489 UV/Vis Detector
  • Manufactured by: Waters