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Waters 1525EF HPLC Pump with Extended Flow Heads

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1525EF HPLC Pump - Extended Flow

Standard analytical flow rates to Semi-preparative flow rates

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Waters® 1500 Series HPLC Pumps have been designed to meet all of your flow range needs, from low flow to preparatory. Exceptional flow rate across all ranges provides you with precision and accuracy, delivering the chromatographic reproducibility that you need to fully automate all your processes. With exceptional solvent blending and pulse-free delivery, the Waters 1500 Series HPLC Pumps can meet your most sensitive application challenges.

The 1525 and 1525 with EF heads are integrated, high-pressure, binary HPLC pumps that feature on-board pulse dampening and efficient mixing. Their pulse-free solvent flow, from analytical to semi-preparative flow rates, makes them ideal for use with highly sensitive detectors.

Designed for use with 2 to 4.6 mm I.D. columns in routine LC and LC/MS applications, these pumps have a programmable flow-rate range of 0.00 to 10.00 mL/min in 0.01 mL increments.

1525EF HPLC Pump with Column Oven.

1525EF HPLC Pump with Column Oven

Optional features:

  • Extended Flow (EF) heads for extended flow rate range up to 22.50 mL/min for use with 10 to 19 mm I.D. columns
  • 1500 Series Column Heater with a temperature range of 20 to 60 °C to maintain accurate, consistent column temperature
  • Integral vacuum degasser to remove dissolved gases from the mobile phase, improving performance and reliability
  • Automated seal wash helps extend the primary seal life for reliable operation
  • FlexInject manual dual injector module for both analytical and semi-preparative injections

1525EF HPLC Pump

The 1525EF HPLC Pump is a high-pressure mixing binary gradient pump that is utilized in many semi-preparative configurations. It provides you with precision and accuracy, delivering the chromatographic reproducibility your lab requires.

Features:

  • 225 μL pump heads deliver up to 22.5 mL/min
  • Optional FlexInject Manual Dual Injector Module for both analytical and semi-preparative injections
  • For semi-preparative applications: 10 to 30 mm inner diameter (I.D.) column separations
  • Maximum operating pressure: 6000 psi up to 22.5 mL/min

 

Specifications:

OPERATING SPECIFICATIONS
Number of eluents 1515: One
1525: One or two
Programmable flow rate range 0.00 to 10.00 mL/min in 0.01 mL increments
Flow precision ≤ 0.1% RSD or 2 sec SD, six replicates, based on retention time or volumetric measures, at 1.0 mL/min, 1000 to 2000 psi backpressure, PQ test (each pump individually)
Flow accuracy ± 1.0% of setting at 1.0 mL/min or 30.0 μL/min, whichever is greater, using degassed methanol, 1000 to 2000 psi backpressure (each pump individually)
Maximum operating pressure 6000 psi (41,370 kPa, 401 bar)
Operating pressure limits Software programmable high and low pressure limits; user selectable in psi, kPa, bar <
Delay volume (1525) 200 μL (with one GM 150 mixer)
Gradient compositional accuracy (1525) ± 0.5% of setting at 1 mL/min and 1000 psi backpressure (methanol:methanol with propylparaben) with one GM 150 mixer
Gradient compositional precision (1525) < 0.5% RSD of setting at 1.0 mL/min and 1000 psi back pressure (methanol:methanol with propylparaben) with one GM 150 mixer (based on six replicates of compositional accuracy)
Pressure ripple (one pump) ≤ 2.0% at 1.0 mL/min, degassed methanol, at 1000 psi backpressure
Gradient ripple < 1.5% normalized to full scale between 10% to 90%, 1.0 mL/min, and 1000 psi backpressure (methanol:methanol with propylparaben with one GM 150 mixer)
1525μ Binary HPLC Pump
OPERATING SPECIFICATIONS
Programmable flow rate 0.00 to 5.00 mL/min, in 0.01 mL/min increments
Flow precision 2 sec SD, six replicates based on retention time or volumetric measures at 0.2 mL/min, 1000 to 2000 psi back pressure, PQ test (each pump individually)
Flow accuracy 2.0% of setting at 0.1 mL/min using degassed methanol at 1000 to 2000 psi backpressure (each pump individually)
Maximum operating pressure 6000 psi (41,370 kPa, 401 bars)
Operating pressure limits Software programmable high and low pressure limits; user selectable in psi, kPa, bar <
Delay volume 30 μL (without mixer), < 100 μL (with one 50 μL mixer)
Gradient accuracy < 1.0% of setting (typical) at 0.1 mL/min between 10% to 90%, and 1000 psi backpressure (methanol:methanol with propylparaben and one 50.0 μL mixer)
Gradient precision < 0.5% RSD (typical) between 10.0% to 90.0%, 0.1 mL/min, and 1000 psi backpressure (methanol:water with octanophenone and one 50.0 μL mixer)
Pressure ripple (one pump) < 2.0% at 0.5 mL/min, degassed methanol, at 1000 to 2000 psi backpressure
Gradient ripple < 1.5% normalized to full scale between 10.0% to 90.0%, 0.1 mL/min, and 1000 psi backpressure (methanol:methanol with propylparaben) with one 50.0 μL mixer
INSTRUMENT CONTROL
Communications Ethernet, IEEE-488
External control Empower™ Breeze™ or MassLynx™ Software
Event I/O Back of instrument Detachable terminal strip
PHYSICAL / ENVIRONMENTAL SPECIFICATIONS
Physical size (without bottle holder) Height: 30.5 cm (12.0 in.)
Depth: 61.0 cm (24.0 in.)
Width: 43.0 cm (17.0 in.)
Weight 1515: 20.4 kg (45.0 lbs)
1525: 27.2 kg (60.0 lbs)
Wetted surface material 316 stainless steel, sapphire, reinforced fluorocarbon polymer seals, carbon-reinforced Tefzel <
Acoustic noise 70 dB(A)
Operating temperature range 4 to 40 °C
Operating humidity range 20% to 80%, non-condensing
1500 Series Manual Injector (Applicable for 1515 and 1525) - Rheodyne 7725I Injector
OPERATING SPECIFICATIONS
Inject switch Contact closure, pre-wired
Mounting Integral for 1500 Series HPLC pumps
Sample holdup Zero
Flow during switching Continuous, make-before-break
Injection Partial or full loop
Loop size 20 μL (standard)
Changeable (5-, 50-, and 200-μL loop supplied)
Wetted materials 316 ss, ceramic, inert polymers
Waters 1500 Series Column Heater
OPERATING SPECIFICATIONS
Set point temperature range 20 to 60 °C; set ≤ 5 °C above ambient temperature
Temperature accuracy ± 0.80 °C
Temperature precision ± 0.25 °C
Pre-column heating of mobile phase ± 0.50 °C of column temperature up to 5.00 mL/min
Pre-heat tube volume Approximately 35 μL
Column capacity Up to four 7.8 mm x 300.0 mm without guard columns
Two columns with guard columns
PHYSICAL SPECIFICATIONS
Dimensions Height: 43.0 cm (17.0 in.)
Depth: 35.6 cm (14.0 in.)
Width: 15.2 cm (6.0 in.)
Weight 5.9 kg (13.0 lbs)
ELECTRICAL SPECIFICATIONS
Input voltage range 120/240 VAC
Input frequency range 50/60 Hz
INSTRUMENT CONTROL
Communications Ethernet, IEEE - 488

 


  • Model: 1525EF HPLC Pump
  • Manufactured by: Waters

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