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Waters SFC Prep 150 AP System

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SFC Prep 150 AutoPurification (AP) System

Please contact our Sales Team for a quote.

Designed for automated UV and/or MS-triggered purification

The SFC Prep 150 AP System is the first and only commercially available preparative SFC system offering automated sample handling, column switching, fraction collection and tracking in an easy-to-use, open bed format.

The SFC Prep 150 AP System provides routine compound purification for drug discovery labs using the MassLynx Software with FractionLynx Application Manager, offering fast separations, high resolution, and high throughput. The inherent benefits of SFC, combined with Waters’ world-renowned service and support provides users with a robust, cost-effective solution to any purification lab.

Key Features:

  • Offers automated sample handling, column switching, fraction collection, and tracking in an easy-to-use, open bed format
  • Achieve fast separations, high resolution, and high throughput
  • Attain routine compound purification for drug discovery labs, using the Waters MassLynx/FractionLynx Application Manager
  • Benefit from a robust, cost-effective solution to any purification lab, when combined with world-renowned service and support from Waters

Recommended Use: For drug discovery labs needing fast separations, high resolution, and high throughput in routine compound purification.

Automate the purification process

The SFC Prep 150 AP System automates the purification process by linking analytical pre-screen, purification and fraction re-analysis with AutoPurify in an “open access” setting.

SFC systems utilize liquid carbon dioxide (CO2) as its main mobile phase in combination with one or more organic solvents resulting in faster equilibration, lower pressure drops across the column, solvent reduction, and decreased cost per sample. The process is reproducible and applicable to a wide range of compounds relevant in the pharmaceutical, life sciences, chemical materials, food, and environmental markets.

Column Switching Fraction Collection.

Automated sample handling, column switching, fraction collection, and tracking in an easy-to-use, open bed format.

Moving SFC forward

The SFC Prep 150 AP System comes equipped with modules to help with routine compound purification, including:

  • QGM Low Pressure mixing Quaternary Gradient Pump: This pump delivers flowrates up to a maximum of 150 mL/min as serves as the co-solvent delivery device for this system.
  • P200X CO2 Pump: This is a high-pressure delivery pump having dual stainless-steel heads with a cam driven sapphire piston assembly; self-priming check valves; presser sensor; pressure gauge; brushless motor; and a rupture disc assembly. The pumps design lends itself to control based upon feedback from the pressure sensor and mass flowmeter.
  • 3767 Sample Manager: This module provides injection and collection capability in an open bed format. The system includes the fume hood assembly for the 3767 for CO2 management. Injection and collection racks must be ordered for the system. The specific racks required will be determined by your needs and requirements.
  • Analytical-2-Prep Column Oven: A thermal control module, the Analytical-2-Prep Column Oven's unique drawer design gives the user unprecedented flexibility, enabling the use of 10 mm, and 20 mm diameter columns simultaneously.

Waters 3767 Sample Manager

Waters 3767 Sample Manager

Optional modifier stream injection

The Modifier Stream Injector’s function is to inject one sample at a time directly into the co-solvent flow. This device allows for settable air gaps on either side of the sample, which function as a buffer between the solvent and the sample decreasing dilution. There are two benefits to the MSI:

  • The sample is injected into the organic portion of the mobile phase (co-solvent) prior to mixing with CO2. The idea is to mitigate the diluent affect by introducing the sample without affecting the overall strength of the mobile phase, and maintaining the programmed solvent percentages throughout the injection and run. Modifier-stream injection provides improved peak shape and resolution, allowing for larger injection volumes, and higher loading (image 1).
  • It allows for Stacked Injections. Stacked injections reduce the time between injection cycles and minimize solvent usage. Stacked injections also significantly improve throughput by utilizing all of the available chromatographic space for continuous separation and purification. Usually, injections are made while an already injected sample is on (or eluting from) the column (image 2).

Chromatograms showing how cycle time is determined

Chromatograms showing how cycle time is determined based on a scouting run (A) and then applied to a set of stacked injections (B). The cycle time is indicated in blue, and is less than 2 minutes. The total run time is indicated in red, and is approximately 4 minutes.

Flavor compound using mixed stream

2 mL injections (40 mg loading) of flavor compounds using mixed-stream (top) and modifier-stream (bottom) injection techniques at 10% co-solvent method conditions.

Specifications:

System Features
Maximum pressure range 300 bar (4350 psi)
Cooling Circulating coolant
Total flow rate range (CO2 + Co-solvent) 20.0 to 150 mL/min
Recovery No Ketoprofen detected, therefore carry over is less than 0.01%
Individual component specifications may allow operation outside the total system defined parameters. The system is designed and tested only for the ranges defined in system specifications.
P200X Pump
Ambient operating temperature 15 to 40 °C
Ambient operating humidity 20 to 80% relative humidity at 40 °C
Pressure rating 10,000 psi (680 bar)
Maximum operating pressure 8700 psi (592 bar)
Flow range 20 to 200 g/min
Wetted materials 316 stainless-steel, 440C, 17-4PH stainless-steel, sapphire, GFPM, PTFE, AL
Co-Solvent Pump (QGM)
Number of solvents Four solvents selected by the Solvent Select Valve (SSV)
Solvent conditioning Sparging one channel per solvent
Operating flow rate range 0.50 to 150.00 mL/min, in 0.01 mL/min increments
Maximum operating pressure 6000 psi up to 100 mL/min, roll-off to 5000 psi at 150 mL/min
Plunger seal wash Integral, active, programmable
Delay volume <6.5 mL
Compressibility compensation Automatic and continuous
Priming Self prime from solvent container on floor with dry lines
Compositional accuracy ±3% absolute (full scale) from 5% to 95%; and from 1 to 150 mL/min (methanol or water step gradient) at 600 psi
Compositional precision Retention time variation <0.15 min SD (uracil/caffeine; 1, 5, 20, 50, 150 mL/min; 70:30 water/methanol dial-a mix)
3767 Sample Injection And Fraction Collection
Sample capacity

Injection: Up to 15 microplates (up to 384 well)
Up to 72 vials
Up to 544 test tubes

Collection: Up to 480 test tubes
Up to 12 deep-well plates
Up to 128 28 mm vials
Syringe delivery accuracy >99.0% at full stroke with 1000 µL syringe
Carryover <0.05%
Injection volume Preparative: 1 mL standard (fixed loop), (0.024 in valve passages)
System pressure ratings 6000 psi High pressure side, including injector
100 psi Collection side and syringe/needle wash
Wash pump >30 mL/min flow through standard tubing and needle; two wash solvents.
Cycle controlled by “Wash Factor” setting
Available loops 1.0 mL (standard preparative) 2.5 mL, 5.0 mL, 12.5 mL, 30.0 mL
Supported syringes 1.0 mL (standard) 2.5 mL, 5.0 mL, 12.5 mL
Injection cycle time <30s based on standard wash cycle
Control software MassLynx v4.2 or higher
Physical / environmental

Without hood

  • Width: 959 mm (37.75 in)
  • Depth: 629 mm (24.75 in)
  • Height: 1020 mm (40 in)

With hood

  • Width: 959 mm (37.75 in)
  • Depth: 629 mm (24.75 in)
  • Height: 1060 mm (41.75 in)
[Optional] Modifier Stream Injector
Default syringe 5 mL
Default loop size 2 mL, (Optional 5 mL, 10 mL)
Injection volume range 0.010 mL (10 µL) to 9.5 mL (9500 µL)
Sample delivery precision Less than 1.0% RSD; 0.75 mL injection volume, 2.0 mL loop
Injection linearity R2 greater than 0.995 using 20–75% of loop volume (2 mL loop)
Sample carryover No Ketoprofen detected, therefore carry over is less than 0.01%
Primary wetted materials 316 stainless-steel, PEEK, PTFE, borosilicate glass
Prep Column Oven
The Prep Column Oven is a thermally controlled module designed for use with preparative SFC columns.
Temperature range Ambient +5.0 to 70.0 °C
Maximum heat rate Approx 6.0 °C/min
Temperature accuracy ±0.5 °C
Maximum column specification 1 to 6, 20 x 250 mm columns
2998 Photodiode Detector
Wavelength range 190 to 800 nm
Light source Prealigned, Intelligent Technology Deuterium lamp
Wavelength accuracy ±1.0 nm
Linearity range <5% at 2 AU (Propylparaben 257 nm, 10 mm cell)
Optical resolution 1.2 nm
Noise, wet ≤60 μAU (254 nm, 2 Hz, 1 s TC, 3.6 BW res, 10 mm analytical cell)
Drift, dry ≤5000 μAU/h (2 h warm-up, constant temperature and humidity at 230 nm, 3.6 BW res, 2 Hz)
Data rate Up to 80 Hz
Path length 3 mm (semi prep)
Cell volume 18.3 μL (semi prep cell)
Pressure limit 6000 psi (408 bar)
Wetted materials 316 stainless-steel, PEEK, fused silica
Line voltage 100 to 240 VAC
[Optional] Isocratic Sample Manager (ISM)
Number of solvents One
Solvent conditioning One integrated, vacuum degassing channel
Primary and accumulator check valves Passive
Pump seal wash Equipped with a wash system to flush the rear of the high pressure seal and the plunger
Compressibility compensation Automated and continuous with no user intervention required
Priming Automated priming at flow rates of 4 mL/min
Flow accuracy 1.0% of set flow at 0.5 to 2.0 mL/min. Backpressure: 1000 psi ±20% with degassed MeOH using either a volumetric or mass based method
Flow precision <0.075 %RSD or <0.02 minutes SD, whichever is greater based on six replicate injections
[Optional] Acquity QDA Mass Detector
Acquisition modes Full scan MS
Selected Ion Recording (SIR)
RADAR An information-rich acquisition approach that allows you to collect highly specific quantitative data for target compounds while providing the ability to visualize all other components
Mass range 30 to 1250 m/z
Scan speed Automatically-optimized for enhanced data quality for acquisition rates of up to e.g. 10 Hz for m/z 100 to 1000 or 20 Hz for m/z 50 to 500
Mass accuracy Mass accuracy is better than ±0.2 Da over full mass range
Mass stability Mass drift is less than 0.1 Da over a 24 hour period
Linearity of response The linearity of response relative to sample concentration, for a specified compound, is up to four orders of magnitude from the limit of detection
Ion polarity switching time 25 ms to switch between positive and negative ion modes
SIR acquisition rate Automatically-optimized for enhanced data quality for acquisition rates of up to 100 Hz
Number of SIR channels Up to 1024 SIR channels (32 functions, 32 channels per function) can be monitored in a single acquisition
Mass resolution Automated mass resolution control (0.7 Da) for constant data quality
SIR sensitivity (ESI+) A 100 pg on column injection (5 μL of 20 pg/μL) of sulfadimethoxine, with a mobile phase flow rate of 800 μL/min will give a chromatographic signal-to-noise for m/z 311 greater than 2000:1 (400:1 with integrated diaphragm backing pump)
SIR sensitivity (ESI-) A 50 pg on column injection (5 μL of 10 pg/μL) of chloramphenicol, with a mobile phase flow rate of 800 μL/min will give a chromatographic signal-to-noise for m/z 321 greater than 300:1 (60:1 with integrated diaphragm backing pump)
SIR signal-to-noise definition Signal is defined as the height of the chromatographic peak of interest and noise is defined as the RMS of a continuous section of the mass chromatogram
Automated Back Pressure Regulator (ABPR)
The ABPR valve assembly is motor driven and temperature controlled for cooling during depressurization. A built-in pressure sensor provides closed loop feedback for control and pressure alarm monitoring.
Maximum flow rate 150 mL/min
Maximum pressure 4400 psi (300 bar)
Heat Exchangers
After exiting the Automated Back Pressure Regulator, this heat exchanger is used to ensure a phase separation in the mobile phase between the CO2 and the co-solvent. This “trailing” Heat Exchanger is used to vaporize the CO2 from the mobile phase so that the Gas Liquid Separator is collecting only a sample-enriched solvent mixture.
Pressure rating 10,000 psi (680 bar)
Wetted material 316 stainless-steel
Gas-Liquid Separator (GLS)
Collecting in the same tubes or racks is achieved by eliminating the majority of the carbon dioxide, directing only the liquid phase along with the collected fraction to the collector.
Total flow rate range 20 to 150 mL/min
Co-solvent max flow range 75 mL/min
Operational pressure range (Optimal) 0 to 100 psi (0 to 6.8 bar)
50 psi (3.4 bar)
Wetted materials 316 stainless-steel, PEEK, borosilicate glass
Software Control
External control MassLynx v4.2
External communication Ethernet and RS-232 communications
Event input/outputs Rear panel contact closure and/or TTL inputs/outputs
Operating system Windows 10


  • Model: SFC Prep 150 AP System
  • Manufactured by: Waters

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