Waters ACQUITY UPLC M-Class System with HDX Technology

ACQUITY™ UPLC M-Class System with HDX Technology

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The ACQUITY UPLC® M-Class System provides reliable high resolution chromatographic separations optimized for columns from 75 μm to 1.0 mm internal diameter. The system’s high pressure, direct (non-split) solvent and sample management system is optimized to minimize dispersive and adsorptive losses. The new sample manager and complementary valve and trapping management module delivers maximum sample recovery with superior chromatographic resolution through efficient use of sub-2-µm particles for a variety of system and column configurations to provide extensive application flexibility.

Quantify changes in protein conformation with confidence

Monitoring protein conformation can be challenging. With the ACQUITY UPLC M-Class System with HDX Technology, your lab can leverage UPLC separations and high-resolution MS (HRMS) to answer important questions about protein conformation through the power of hydrogen deuterium exchange (HDX), like drug candidate binding to a protein, biopharmaceutical product development, protein-protein interactions, and intellectual property.

The system provides HRMS and intuitive informatics for confident measurement of changes in protein conformation by controlling important experimental parameters. With the ACQUITY UPLC M-Class System with HDX Technology, your lab can confidently identify changes in protein conformation by stringently controlling the labeling reaction, and efficiently processing and separating the sample before mass analysis.

Key Features:

• Rapid chromatographic resolution at 0 °C with UPLC technology
• Temperature-controlled environment for trapping and analytical columns
• Repeatable peptic digestion with Waters Enzymate BEH Pepsin Column, capable of operation up to 15,000 psi
• Easy access injection port for flexibility with manual or robotics injection
• Integrated with Waters high resolution MS for precise determination of Deuterium label
• Intuitive, interactive DynamX HDX Data Analysis Software to shorten the time between experiment and answers

Recommended Use: Answering important questions about changes in protein conformation.

Accurately, reproducibly, and easily perform protein conformation studies

The unprecedented system-level design integrates all the steps necessary to accurately, reproducibly, and most of all, easily, perform protein conformation studies. The system and its DynamX HDX Analysis Software remove the barriers to adopting high resolution LC and MS for routine studies of protein conformational changes, making hydrogen deuterium exchange with MS (HDX, HDX MS, HXMS, or H/D exchange) a core competency for your laboratory.

DynamX HDX Data Analysis Software automates HDX-MS data processing, displaying the deuterium uptake results in a simple and interactive software interface.

Higher efficiency separations

Carefully controlling temperature-sensitive parts of the HDX workflow ensures that the conformational changes are captured and not missed. UPLC separations of up to 15,000 psi take place within the cooled environment with the added advantage of sub-2-µm particles for enhanced chromatographic resolution and speed. Maintaining temperatures at 0±1 °C means that back-exchange is kept to an absolute minimum with the ACQUITY UPLC M-Class System with HDX Technology.

Once the HDX reaction has been chemically quenched and manually introduced, the HDX Manager directs the sample to the pepsin column for peptic digestion, or directly into the mass spectrometer (MS) for global analysis. The sample is then trapped for a rapid UPLC analytical separation.

Using UPLC, high resolution MS and IMS can dramatically decrease run times while delivering high peak capacity separations. This orthogonal approach yields faster separation times, higher throughput, and unequivocal spectral information.

Specifications: