Waters Fraction Manager - Analytical

Fraction Manager - Analytical

Please contact our Sales Team for a quote.

Uniquely designed to accurately collect fractions from high-efficiency UPLC separations

Efficiency gains realized by ultra-performance liquid chromatography (UPLC) and ultra-high-performance liquid chromatography (UHPLC) separations often get lost with traditional slow-speed, high-volume HPLC-grade fraction collectors.

Minimize fraction loss and carryover to better manage low volume peaks and efficiently collect small amounts of material for further assays with the Waters Fraction Manager (WFM-A), an analytical-scale fraction collector for UPLC systems. Waters has designed the WFM-A to provide high purity and recovery, confidently minimize sample loss, and reduce carryover of your complex mixtures and precious samples.

Overview

• Designed specifically to collect the narrow and closely eluting compounds found with UPLC separations.
• Developed on the ACQUITY UPLC platform, the industry standard for innovative and reliable technology, the WFM-A integrates seamlessly into all Waters analytical UPLC/UHPLC and HPLC systems.
• Biocompatible when paired with ACQUITY Premier System with MaxPeak High Performance Surfaces (HPS) Technology collection needle and inert flow path to cool fractions for biopharma applications.

Recommended Use: Ideal for accurately collecting fractions in pharmaceutical, biopharmaceutical, impurity isolation, metabolite, and natural product applications.

Purposefully designed for high-efficiency, high-purity collections

The WFM-A overcomes the peak purity challenges of typical fraction collectors and includes features like:

• Low peak dispersion: The low volume, rapid switching valve reduces dispersion and allows the precise collection of narrow UPLC peaks.
• Sample recovery: To further increase recovery of your smallest fractions, the advanced needle design focuses flow and prevents precious sample droplets from forming on the tip.
• Temperature control: The sample compartment is precisely controlled at a range of 4–40 °C to accommodate thermally labile samples.
• Biocompatibility: The WFM-A inert flow path allows effortless integration into your UPLC or HPLC system, accommodating all biomolecule applications.
• System versatility: Specialized needles are designed to accurately dispense fractions for UPLC flow rates of 2 mL/min or higher HPLC flow rates of 5 mL/min.

Waters Fraction Manager - Analytical

Novel design for less carryover, maximum recovery, and higher purity

Fraction purity is a major concern when performing analytical fraction collection due to the inherent small volume application work. Classic fraction collector designs trap a portion of the fraction in the dispensing tube when the valve closes. The trapped portion is then collected with the next fraction when it is dispensed typically referred to as “crosstalk.”

The innovative fluidic design of the WFM-A minimizes carryover risk. The syringe can be programmed to dispense a small amount of rinse solvent through the dispense needle after a fraction is collected. This rinse can be directed either to waste, or into the current fraction vessel increasing fraction purity by reducing crosstalk.

Interactive control showing rinse fluidics with the Waters Fraction Manager - Analytical.

Better peak integrity with minimal band broadening

The negligible volume of the WFM-A fraction valve, combined with optimized tubing volumes between the detector and the valve, minimizes band spreading of the fractions. The figure shows that the WFM-A fraction valve peak shape superimposes the straight bypass, whereas the other valves demonstrate much greater dispersion.

The WFM-A effectively collects what you want, when you want it.

WFM-A dispersion performance shows better peak integrity as the valve peak shape superimposes on the straight bypass – unlike the greater dispersion of the other valves.

Purification Sample Management System Components:

• 2707 Autosampler – A flexible autosampler that manages sample injection from both microtiter plates and/or vials. It can also be configured for biocompatible or preparative scale injections.
• 3767 Sample Manager (Injector/Collector) – A high-capacity sample processing system that easily and automatically manages concurrent sample injection, collection of peak fractions, and fraction analysis on a single HPLC or LC-MS platform.
•  Manual FlexInject Module – The FlexInject includes analytical and preparative valves with a manual selector valve. It features the reliable 7725 analytical injector with a 100 µL sample loop and the 3725i preparative injector configured with a 5 mL sample loop. Additional loop sizes can be ordered separately.
• Manual Prep Injector Kit – The Prep Injector was built on the Flex Inject platform. It features the reliable 3725i preparative injector comes standard configured with a 5 mL sample loop and a 10 mL sample loop in the start-up kit. Additional loop sizes can be ordered.
• Preparative Chromatography Rack – The Waters Preparative Chromatography Rack (PCR) incorporates manual small-scale and large-scale injectors with manual fraction collection. You can use the scale select valve to alternate between the small-scale and large-scale flow paths.
• Waters Fraction Manager – Efficiently isolate and recover small samples for additional analyses and increase recovery with the Waters Fraction Manager, a fraction collector designed for use with UPLC systems.
• Waters Fraction Collector III – Precisely perform fraction collection in chromatography with the easy-to-use Waters Fraction Collector III, which can be used alone or as part of a Waters sample purification system for even greater sample collection capacity.

Recommended Use: For flexible, easy-to-use purification sample management.

Specifications: