A fast, versatile HPLC column packing material, Hamilton PRP-X300 columns rapidly and easily separate closely related alcohols and organic acids without column heating. The sulfonated poly(styrene-divinylbenzene) support separates samples by a mixed mode mechanism.
The three separation modes are:
1. Hydrogen Bonding | The attraction and retention of sample compounds by the negatively charged sulfonate group |
2. Reversed Phase | The interaction and retention of the sample compounds by the nonpolar polymeric support |
3. Ion Exclusion | The process in which ionized samples are excluded from the pores of the support and elute first, while the non-ionic compounds elute later |
