Dr. Maisch Exsil 80 SAX Analytical HPLC Column, 5 µm, 80 Å, 4.6 x 250 mm, 1/pk - 5136495.s2546

ExSil® 80

Dr. Maisch Exsil 80 SAX Analytical HPLC Column, 5µm, 80Å, 4.6mm I.D. x 250mm Length, 1/pk

Product Number: 5136495.s2546

• 80 Å
• 0.51 cc/g
• 226 m2/g 150 m2/ml
• Type A Silica and Bonded Phases
• Alternative to Spherisorb
• Available in 1.5, 3, 5 and 10 μm particle sizes
• Exsil 80: Columns with a pore size of 80 Å Angstroms (8 nm)

The Exsil 80 range has a higher surface area and greater retention than the original Exsil 100 which in some instances provides a better alternative to Spherisorb.

• Stationary Phase: SAX columns contain a solid support (usually silica or a polymer) with covalently attached strong anion exchange groups, such as quaternary ammonium.
• Strong Anion Exchange: SAX refers to "strong" anion exchange, meaning the functional groups on the stationary phase are permanently charged, even at low pH. This contrasts with weak anion exchange (WAX) where the charge can be pH-dependent.
• Anion Binding: When a sample containing negatively charged molecules (anions) is passed through the column, these anions will bind to the positively charged stationary phase.

Applications:

SAX chromatography is used in various fields, such as:

• Proteomics: Enriching and separating phosphopeptides and glycopeptides.
• Environmental analysis: Separating and quantifying anions in environmental samples.
• Pharmaceutical analysis: Analyzing nucleotides, organic acids, and other charged molecules.
• Biomolecule analysis: Separating and analyzing proteins, peptides, and other biomolecules.
• Food science: Analyzing sugars and other carbohydrates.
• Orthogonality: SAX chromatography is often used in combination with other separation techniques, like reversed-phase chromatography (RP), to achieve better separation and identification of complex mixtures.

Specifications: