Imtakt Unison UK-Amino MF 2mm ID, 75mm Length, 3um,Aminopropyl, Metal-Free Column - UKA23F

Unison UK-Amino 3μm Column, Durable against water elusion

Unison UK-Amino MF (Aminopropyl) 3μm, 75mm x 2mm, 130Å Analytical Metal-Free HPLC Column, Pressure Limits of up to : 50MPa, 500 bar, 7,500 psi

Imtakt Product Number: UKA23F

Download the Metal-Free Column Brochure

Column Specifications:

High quality long-life Amino column for carbohydrate or Hilic separations.

The Unison Family of columns provide a wide range of high quality reverse phase and normal phase columns. The quality of our material provides high efficiency with low backpressure and 100% aqueous stability. Peak shape is also improved with the use of our polymeric end-capping technology. The Unison series of columns are available with C18, C8, Phenyl, Amino and Silica stationary phases to meet a wide range of chromatographic needs.

• Pure spherical porous silica 3µm particle Aminopropyl phase
• High efficiency 3µ columns with mono-dispersed packing for low backpressure
• Normal phase aminopropyl material ideal for analysis of sugars and carbohydrates
• World class reproducibility
• HT and UP hardware able to operate in pressures up to 1000 bar
• High efficiency and available short column dimensions are ideal for high throughput applications
• Aqueous to non-aqueous Normal Phase separation
• LC-MS applicable
• Unique ligand binding strategy provides dramatically longer lifetimes and lower bleed compared to other amino columns

Aqueous durable silica-based aminopropyl columns have been used for a long time as a normal phase column for carbohydrate separation. However, these aminopropyl columns have a fatal flaw: "column bleeding" or the rapid deterioration in retention as a result of ligand desorption under aqueous elution.

Unison UK-Amino offers a different approach from conventional columns: high durability against aqueous eluent. As the above chromatogram demonstrates, conventional columns experience a significant decline in retention when an aqueous mobile phase elutes through the column. UK-Amino, on the other hand, does not show any change in separation or retention. This is a significant development in the history of aminopropyl columns.

UK-Amino's design not only provides analytical power, but the 3um particle high-resolution column has other benefits, including the minimization of LC-MS and LC-ELSD noise levels. UK-Amino can be applied to aqueous normal phase conditions, and separation optimization is possible while comparing to ODS columns using reversed-phase mode. One can expect significant results from this normal phase column of UK-Amino.

Unison UK-Amino Metal-free HPLC Columns

Improving peak shape for chelating or phosphoric compounds

2 mm I.D. - 30-250 mm Length - PEEK / SS316 - UHPLC compatible

• Metal-free (PEEK) internal structuren 
• UHPLC compatible metal housing
• Ideal for chelating compounds
• Ideal for phosphoric compounds
• LC-MS compatible 2mm I.D.
• Wide selection of stationary phases

This metal-free column is an Imtakt original design. The entire internal surface of these columns are made with PEEK material, including the frits, and is therefore extremely inert with respect to chelating compounds. The outer portion of these columns are made from stainless steel (SS316) making them suitable for extremely high pressures, and are UHPLC compatible. 

Phosphoric compounds can interact with the exposed metal surfaces within the column causing peak tailing, as seen in the figure to the left.

Imtakt's metal-free columns are designed to specifically improve these poor peak shapes by decreasing unwanted secondary chelating effects.

Improving peak shape of chelating or phosphoric compounds
Chelating and phosphoric compounds often have difficulty with poor peak shape and/or sensitivity due to undesired interaction between metal parts in the column and the structure of these compounds. Imtakt's UHPLC compatible metal-free columns, which are an Imtakt original design, will improve these
problems and are available in a wide range of stationary phases.

Range of Unison UK-Amino 3µm Columns, Product Numbers:

Column Guard System for Unison UK-Amino:

All stationary phases can also be made in the following internal diameters, please contact our Sales Team with your specificatons: Nano: 0.02mm, 0075mm | Capillary: 0.1mm, 0.3mm, 0.5mm | Semi-Prep: 20mm, 28mm.

This Column is also Available in 5μm!

• Imtakt Unison US-Amino

Additional Imtakt Columns Available

• Imtakt Scherzo
• Imtakt Intrada
• Imtakt Cadenza
• Imtakt Presto
• Imtakt Unison
• Imtakt DACAPO
• Imtakt Guard