Supercritical Fluid Chromatography (SFC) - 2.5Cholester (Cholesteryl)
Nacalai Tesque COSMOSIL 2.5Cholester Analytical SFC Column, 2.5µm, 130Å, 2.1mm I.D. x 150mm Length, 1/Pk
Product Number: 13752-31
Longer retention and better separation than C18
Supercritical Fluid Chromatography (SFC) has become more attractive because it offers some advantages over HPLC, such as high speed, unique selectivity and environmentally friendly separations. Many conventional normal-phase stationary phases, such as diol, amino and cyano, have been used for SFC applications. However, these phases present limitations for separations. COSMOSIL SFC Columns have been developed to enhance the capability of SFC separations.
Nacalai Tesque has developed columns specially designed for SFC in collaboration with Nacalai USA and Pfizer, Inc. Global R&D: COSMOSIL HP, PY (equivalent to 2-ethylpyridine) and Quinoline. In addition to these, our HPLC columns Cholester and PBr have been tested for use with SFC.
Cosmosil® Cholester Columns
Cholester is a silica-based reversed-phase column using cholesterol as the bonded phase. It has similar hydrophobicity to C18 (ODS) and, with superior stereoselectivity, it is suitable for compounds with similar hydrophobicity but slightly different molecular shape. Our COSMOSIL series features many packing materials, such as πNAP, NPE, PYE and PBr, that can separate compounds difficult to separate using ODS. However, these materials utilize several kinds of molecular interaction, so it is necessary to reconsider analysis conditions. Designed for ease of use, Cholester can be used under solvent and other analytical conditions identical to ODS.
- Better selectivity for cis-trans isomers, polyphenols, and natural products
- Cholesterol-bonded reversed-phase column
- Usable under the same conditions as C18 columns
- Available particle sizes: 5 μm, 2.5 μm, 2.6 μm core-shell
COSMOSIL SFC Column Series
Packing material | PY | HP | Diol | Cholester | πMAX | PBr |
---|---|---|---|---|---|---|
Silica gel | High-purity porous spherical silica | |||||
Average particle size | 3, 5 µm | 5 µm | 5 µm | 5 µm | 5 µm | |
Average pore size | 120Å | |||||
Stationary phase | Pyridinyl | 3-Hydroxyphenyl | Diol | Cholesteryl | Pyrenylethyl | Pentabromobenzyl |
Endcapping treatment | Near-Perfect Treatment |
Available in the Following Configurations:
- 2.5Cholester - 2.5 µm UHPLC Column
- 3Cholester - 3 µm HPLC Column
- 5Cholester - 5 µm HPLC Column
Column Specifications:
Product description | Cosmosil® 2.5Cholester Packed Column (for SFC) 2.1mmx150mm |
---|---|
Number / Quantity | 1 pc |
Manufacturer | Nacalai Tesque |
Brand name | Cosmosil |
Product type | SFC Column |
length | 150 mm |
inner diameter | 2.1 mm |
Particle size | 2.5 µm |
modification | Cholesterol |
Modification (manufacturer) | Cholester |
Pore size | 130 Å |
USP Class | L101 |
Carbon content | 20.0% |
Specific surface area [m2/g] | 330.0 |
Column type | Analytical Column |
Hardware material | stainless steel |
Hardware Type | Ready-to-use column |
pH minimum | 2.0 |
pH maximum | 7.5 |
Separation technology | Supercritical Fluid Chromatography (SFC) |
technology | SFC |
matrix | Silica |
Particle morphology | spherical, fully porous |
Additional Column Sizes are available - Please contact our Sales Team with your requirements.
Cosmosil Columns Online Catalogue:
- Model: 13752-31
- Manufactured by: Nacalai Tesque