Tosoh TSKgel Tresyl-5PW Column, 10μm, ID 6 mm x L 4 cm - 14455

TSKgel Tresyl-5PW Column, 10μm, ID 6 mm x Length 4 cm

Unlike other TSKgel affinity columns, TSKgel Tresyl-5PW, which is derivatized with the 2,2,2-trifluroethanesulfonyl ligand, requires activation with a user-selected ligand containing amino, thiol, phenol, or imidazole groups.  The resulting structure is literally a "custom" affinity ligand with excellent pH stability and minimal ligand loss due to leaching. TSKgel Tresyl-5PW readily reacts with amino or thiol groups to form stable covalent alkylamines or thioethers.

Applications include: glycoproteins, antigens, etc.

Structure: Pore size (mean): 1,000 Å  Particle size (mean): 10 µm  pH stability: 2.0 - 12.0  Ligand conc.: ca. 20 µmol/mL resin Adsorption capacity: > 60 mg/g dry resin  (coupling capacity w/ soybean trypsin inhibitor) Exclusion limit (base resin, estimate): < 10,000,000 Da globular proteins Active group:  tresyl

Purification of peroxidase on concanavalin A  coupled to TSKgel Tresyl-5PW