Waters ACQUITY UPLC Peptide BEH C18 Method Validation Kit, 130Å, 1.7 µm, 2.1 mm X 100 mm, 1K - 15K, 3/pk
Product Number: 186004896
The method validation kit (MVK) contains three columns each packed with a different lot of material. As the premier column for method development, ACQUITY Premier BEH C18 columns provides you with the most universal column choice for high performance separations. MaxPeak High Performance Surface hardware technology significantly reduces unwanted analyte/surface interactions that can lead to poor peak shape and losses in signal intensity. The trifunctionally bonded BEH particle gives you the widest usable pH range (pH 1-12), which is ideal for methods development.
PST (Peptide Separation Technology)
peptide mapping. Reversed-phase (RP) chromatography has become the separation mode of choice due to its relatively high resolving power and outstanding quantitative (UV) and qualitative (ESI-MS) information. Waters Ethylene Bridged Hybrid (BEH Technology) and new Charged Surface Hybrid (CSH™) Peptide Separation Technology columns provide capabilities that help address demanding application needs.
BEH (Ethylene Bridged Hybrid) Technology
Ethylene Bridged Hybrid (BEH) particle technology ensures maximum column performance and longer column lifetimes under all chromatographic conditions. The hybrid particle technology offers many advantages over conventional silica-based particles, including the ability to control silanol activity for better reproducibility, peak shape, and efficiency. The BEH particle is created from two high purity monomers: tetraethoxysilane (TEOS) and bis(triethoxysilyl) ethane (BTEE), which results in highly stable, pH resistant, and mechanically strong particles that set the performance standard for method development. BEH particle technology allows for seamless transfer from analytical to preparative separations.
The advantages of BEH columns include:
- Rugged hybrid organic/inorganic base particle
- Improved peak shape for bases-low silanol activity
- Wider operating pH range for method development flexibly
- Mechanical strength for maximum column efficiency at UHPLC operating pressures
- Available Particle Sizes: 1.7, 2.5, 3.5, 5, and 10 µm
- Column Chemistries: C18, C18 AX, Shield RP18, C8, Phenyl, Amide, Z-HILIC, HILIC
Specifications
Chemistry | C18 |
Separation Mode | Reversed Phase |
Particle Substrate | Hybrid |
pH Range Min | 1 pH |
pH Range Max | 12 pH |
Maximum Pressure | 15000 psi (1034 Bar) |
Endcapped | Yes |
Silanol Activity | Low |
Molecular Weight Range Min | 1000 |
Molecular Weight Range Max | 15000 |
Particle Shape | Spherical |
Particle Size | 1.7 µm |
Endfitting Type | Parker-style |
Pore Size | 130 Å |
QC Tested | Peptide |
Format | Method Validation Kit |
Surface Area | 185 |
System | UHPLC, UPLC |
Particle Technology | BEH |
USP Classification | L1 |
Inner Diameter | 2.1 mm |
Length | 100 mm |
Carbon Load | 18% |
UNSPSC | 41115709 |
Application | Peptide |
Brand | ACQUITY UPLC |
Product Type | Column Kits |
Units per Package | 3 pk |
- Model: 186004896
- Manufactured by: Waters