Waters Alliance HPLC Bioseparations System

Alliance HPLC Bioseparations System

Please contact our Sales Team for a quote.

When Nothing Else Matters More than Accurate Results

The Alliance® Bioseparations (AllianceBIO) System is an automated, high-resolution HPLC system featuring a titanium/polymeric flow path that is ideally suited for the fractionation and analysis of complex biomolecular mixtures in biopharmaceutical applications. The heart of the AllianceBIO is the 2796 Bioseparations Module designed for robust operation under the harsh salt and pH conditions typical of bioseparations.

In the ever changing world of Biopharmaceuticals, reliable and robust separation technologies are needed to keep pace with this fast growing area of discovery. System accuracy and reproducibility mean everything to scientists who stake their reputations on the results they generate or methods they develop.

The Alliance® HPLC Bioseparations System, a high-precision, high-resolution HPLC system for the fractionation and analysis of complex biomolecule mixtures, is ideally suited to help accelerate the progress of your biotechnology analyses.

The core of the Alliance HPLC Bio System is the Waters e2796 Bioseparations Module. Designed for robust and reliable operation under the harsh salt and pH conditions typical of bioseparations, it features an inert flow path, making it ideal for protein purification, peptide mapping, and nucleic acid analysis.

Key Features:

• Biocompatible flow path (Titanium/PEEK)
• Auto•Blend™ Technology, which blends up to four eluents to perform isocratic, step, continuous gradient, or flow program applications
• Automated SystemPREP routine for rapid unattended system equilibration
• Precise temperature control for enhanced sample stability
• Compatibility with high- pressure and low-pressure column chemistries
• Automated column switching, flow diversion, and fraction management to support comprehensive multi-dimensional separations methods
• Enhanced system monitoring functions (pH, conductivity, composition, valve, and fraction tracking) for methods development and troubleshooting
• Proven day-to-day reproducibility
• Integrated Fluidics for Consistent Performance and Enhanced Reproductivity
• Solvent management

The simplified design of the e2796 Solvent Manager enables the blending and delivering of solvents in a pulse-free, low-dispersion flow path. In addition, Synchronized Composition Control™ (SCC™) of the gra- dient proportioning valve (GPV) ensures that the e2796 Separations Module delivers excellent gradient composition accuracy and precision.
The overall design results in reduced complexity and enhanced overall ruggedness across a flow range that covers two orders of magnitude.

The Waters e2796 Bioseparations Module is the core of the system. Its integrated solvent management and sample management provides consistent performance and enhanced reproducibility for high quality, high-throughput analysis for HPLC, LC/MS, or flow injection-mass spectrometry applications.

Sample management

The design of the e2796 Sample Manager ensures precise sampling – delivering predictable peak areas, and consistent results. Complimenting the simplified design of the solvent flow path, a user programmable needle wash, and selectable “purge-wash-purge” cycle ensures that any part of the system exposed to sample is fully washed, virtually eliminating the risk of sample carryover.

The inherent versatility of the three-axis (X, Y, Z) needle assembly of the e2796 Sample Manager allows for greater applications flexibility. The sample manager has a wide range of sample loops, holding loops, and syringe sizes available and can process samples from a variety of plates, vials, and/or tubes. This variety allows you to customize the configuration of the sample manager according to the needs of your applications.

Auto•Blend Technology

The e2796 Separations Module features Auto•Blend Technology, which enables you to generate gradients automatically without having to manually alter the buffer concentration. Auto•Blend programming technology improves uptime since no premixing of mobile phases is required.

Automated system start-up

The Alliance HPLC Bio System also features SystemPREP – a programmable, automated system preparation routine – for fast, one-touch, walk-up operation. This function performs a series of automated steps, putting the separations module in a condition of complete system readiness for running samples. SystemPREP is useful when you are changing solvents or after extended periods of system inactivity and is a simple-to-use alternative to running the individual functions of Wet Prime, Purge Injector, Equilibrate, and Condition Column.

DO MORE WITH YOUR RESULTS

Waters software solutions enable you to capitalize on the results generated by your system. The Waters Laboratory Informatics Suite includes proven information management solutions that integrate all your lab resources in a consistent, scalable, and seamless application environment.

Empower™ Software provides control of the system, including data acquisition, management, processing, and reporting, all in the market-leading chromatography data software (CDS) platform. Empower Software is scalable from a single workstation to an enterprise-wide CDS solution, and offers advanced features for method development, reporting, and custom calculations, as well as Enterprise Options for advanced data management and automated method valida- tion management.

MassLynx™ Software, for advanced mass spectrometry applications, enables researchers to acquire, analyze, manage, and distribute information. MassLynx Software offers flexible control over complex instrument configurations, and Application Managers that are tailored for specific MS analyses.

NuGenesis® SDMS is a customizable scien- tific information management platform that automatically captures and stores data in a centralized data warehouse. SDMS makes information easier to access, facilitates regulatory compliance, and aids in the administration of intellectual property. SDMS converts scientific results into accessible and valuable corporate knowledge.

LEADING BIOSEPARATIONS CONSUMABLES

When your work involves the analysis of proteins, peptides, and amino acids, Waters has a variety of chemistry consumable solutions for biomolecule separations in areas ranging from proteomics to the validation of well-characterized biopharmaceuticals.

BioSuite HPLC Columns

Designed specifically for bioseparations, Waters’ line of BioSuite HPLC Columns deliver the resolution and recovery of proteins and peptides scientists need. Available in ion exchange, size exclusion, hydrophobic interaction, and reversed-phase modes, BioSuite HPLC Columns are available in analytical as well as “lab scale” prepara- tive dimensions.

Peptide separation technology columns

Peptide Separation Technology is a unique column chemistry that meets the demanding requirements of peptide separations used in a range of applications. Available in 130 Å and 300 Å pore sizes, Peptide Separation Technology columns are available in numerous particle sizes and column dimensions for consistent separations, from high-sensitivity proteomics, to peptide mapping, to high mass load purification. Peptide Separation Technology columns are specifically QC tested with a protein digest to ensure exceptional application performance.