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Waters Fraction Collector III

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Fraction Collector III

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Adaptable and reliable fraction collection

Collecting purified material from your liquid chromatography (LC) system, especially in a user-friendly and cost-effective way, can be a challenge.

Waters Fraction Collector III is an easy-to-use, standalone, or software-controlled fraction collector designed for use with high performance liquid chromatography (HPLC) and LC-MS applications. Compatible with flow rates as high as 300 mL per minute, the Fraction Collector III handles a wide range of adapter racks and related collection vessels. Its flexible design accommodates a variety of fraction sizes, whether you are collecting to microtiter plates or multiple containers.

Overview

  • Multi-purpose functionality in a small footprint
  • Intuitive keypad and view screen make setup easy
  • Review results in real time, or via a post-run results table
  • Compatible with the LC Prep 150 System, LC Prep AutoPurification System, and Modular Systems
  • Remote management with MassLynx Software for mass spectrometry (MS) applications

Recommended Use: For flexible collection of a variety of fraction sizes, designed for use with HPLC and LC-MS applications.

Several modes of collection

Fraction Collector III offers several simple to advanced modes of operation for fraction collection, including time, threshold, volume, manual, and sampling. These modes of operation can be combined to allow for advanced fraction collection, such as pooling of repeated runs into common vessels.

Peak detection and collection using an analog detector input from a liquid chromatography (LC) system permits fully automated collection of the peaks of interest. The diverter valve in the system enables you to collect peaks without extraneous solvent and/or unwanted peaks (which are diverted to waste), maximizing fraction collection efficiency and overall preparative LC system productivity.

For maximum control and usability, you can control the Fraction Collector III from a local keypad or through serial port communication by Empower 3 or MassLynx Software.

Top image: Peak collection based on peak number discrimination allows you to detect up to 99 peaks and designate up to 10 of those peaks for collection. You can program an absolute value above which peaks are detected, and then specify the peaks you want to collect. Bottom image: Peak collection based on slope value discrimination for use when there is a large drift in the baseline. Collection begins when the slope of the signal exceeds the preset value entered by the user and continues until the signal slope falls below the preset value.Top image: Peak collection based on peak number discrimination allows you to detect up to 99 peaks and designate up to 10 of those peaks for collection. You can program an absolute value above which peaks are detected, and then specify the peaks you want to collect. Bottom image: Peak collection based on slope value discrimination for use when there is a large drift in the baseline. Collection begins when the slope of the signal exceeds the preset value entered by the user and continues until the signal slope falls below the preset value.

Top image: Peak collection based on peak number discrimination allows you to detect up to 99 peaks and designate up to 10 of those peaks for collection. You can program an absolute value above which peaks are detected, and then specify the peaks you want to collect. Bottom image: Peak collection based on slope value discrimination for use when there is a large drift in the baseline. 

Integrated instrument control software interface

Control of the Fraction Collector III is accessible through a new instrument control software interface that integrates seamlessly with Empower Software control components. The user can use the interface to configure collection vessels and program collection routines. Real-time feedback from the Fraction Collector III indicates the state of the collection process and the location of the collected fractions.

Multiple collector rack configurations (including custom configurations) accommodate a variety of collection vessels. If the user needs to override collection control, the interface enables software-assisted manual collection through its override function, directly from the instrument control panel.

Integrated control panels offer consistent instrument control interfaces to view data by year, month, and day.Integrated control panels offer consistent instrument control interfaces to view data by year, month, and day.

Integrated control panels offer consistent instrument control interfaces to view data by year, month, and day.

Specifications:

Rack Options
  • Standard test tube (13.0 to 18.0 mm) rack
  • Eppendorf tube rack
  • Scintillation vial (16.5 mm) rack
  • Scintillation vial (27.5 mm) rack
  • Microtiter plate (4 x 96/384) rack
  • Preparative funnel (4 x 32 positions) rack
  • Multipurpose rack
Collection
Methods Time, drop count, drop volume, signal
Nozzle shift time 0.1 s (tube center to center)
Modes of Operation
Simple mode Time 99 min 59 s/tube max.
Drop 9999 count/tube max.
Volume 999.9 mL/tube max.
Signal 9999 count/tube max.
Peak mode End time 999 min max.
Request time 99 min 59 s/tube max.
Monitor time 99 min 59 s/tube max.
Fraction peak Up to 10 out of 99 peaks
Peak discrimination Absolute value 100% max.
Slope 100 mV/min or 10 V/min max.
Window mode Non-window monitor 99 min 59 s max.
No. of windows 10 max.
Start/end time 999 min 59 s max.
Request/monitor time 99 min 59 s/tube max.
Manual mode Learning response Chromatogram signal display
High 0.5 s; low 3.0 s
Sampling mode Playback Replay results of learning
Interval 99 min 59 s max.
Collection time 99 min 59 s max.
Collection volume 999.9 mL max.
Number of repeats 99 max.
Electrical Specifications
Event markers 2 contact point output circuits
Signal input 10 mV or 1 V full scale
External start input On start
External end input On end
External count input On count
Serial interface RS-232C (9-pin connector)
Power requirements 100.00 V AC
115.00 V AC
240.00 V AC
0.23 A max.
Local user interface Operation display 40 characters by 8 rows (LCD w/backlight)
Parameter memory Battery back-up
Remote control PC control with MassLynx and Empower 2 Software
Physical / Environmental Specifications
External dimensions Width: 28.60 cm (11.27 in.)
Depth: 35.90 cm (14.14 in.)
Height: 33.50 cm (13.20 in.)
Weight Approximately 7.00 kg (15.40 lbs)
Temperature range 2 to 40 °C (35 to 104 °F)
Ordering Information
Title Part Number
Waters Fraction Collector III
(Includes one standard rack that holds up to 120 tubes; 12 to 18 mm O.D. x 90 to 180 mm height)		 186001878
Diverter valve options
Divert valve assembly, standard 725000142
Divert valve assembly, low volume
(Recommended for microtiter plate rack)		 725000143
Fittings kit
(Drop tubes and connecting tubing to accommodate different flow rates)		 205000131
Rack options
Microtiter plate rack
(Accepts four microtiter plates, standard, and deep-well)		 725000110
Dropper extension nozzle
(Recommended for microtiter plate rack)		 725000151
Eppendorf tube rack
(accepts up to 120 eppendorf tubes)		 725000145
Scintillation vial rack, 16.5 mm
(accepts up to 120 scintillation vials; 16.5 mm O.D. x 54.0 mm height)		 725000146
Scintillation vial rack, 27.5 mm
(accepts up to 56 scintillation vials; 27.5 mm O.D. x 57.0 mm height)		 725000147
Multipurpose rack 725000113
Preparative funnel adapter rack
(used with preparative funnel adapters – up to four)		 725000107
Preparative funnel adapter, 2 per package
(collects up to 32 fractions; requires tubing to connect adapter to collection vessels)		 725000106
Tubing for preparative funnel adapter, Tygon 50 feet WAT037047
Tubing for preparative funnel adapter, Teflon 50 feet WAT037090
Table-top shelf assembly, for use with prep funnel rack 289000440

 


  • Model: Fraction Collector III
  • Manufactured by: Waters

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