| System Features |
| Dwell volume (total system) |
≤400 μL (includes standard 100 μL mixer) |
| Gradient delay volume |
≤300 μL (includes standard 100 μL mixer) |
| Integrated leak management |
Leak sensors, as standard equipment, compatible with the supported solvents and safe leak handling; drip trays direct all leaks to the front of the instrument and into the waste line |
| Maximum operating pressure |
0 to 1.0 mL/min: 15,000 psi
1.0 to 2.2 mL/min: 15,000–7,800 psi (linear) |
| Super synchronization |
Synchronization between the solvent manager pistons enhances retention time reproducibility |
| Operating flow range |
0.010 to 2.20 mL/min in 0.001 mL increments pH range 2 to 12 |
| Unattended operation |
Full 96-hour diagnostic data displayed through the instrument console software |
| Isocratic Solvent Manager |
| Number of eluents |
One standard, or six with optional solvent selection valve |
| Solvent conditioning |
Two integrated, vacuum degassing channels, one of which is used for the SM-pFTN purge solvent |
| Primary check valve |
Intelligent Intake Valve (i2 Valve) |
| Pump seal wash |
Equipped with a wash system to flush the rear of the high-pressure seal and the plunger |
| Compressibility compensation |
Automatic and continuous with no user intervention required |
| Solvent selection |
Automatic up to six solvents (optional) |
| Flow ramping |
0.01 to 30.00 min to reach 2.0 mL/min |
| Flow accuracy |
±1.0% of set flow at 0.5 to 2.0 mL/min (both water and THF) with back pressure at 2000 psi ± 10% |
| Flow precision |
<0.075% RSD or <0.020 min SD, whichever is greater based on six replicates of a polystyrene standard at 10-μL injection volume, 0.5, 1.0, and 1.5 mL/min, 100% THF, ACQUITY APC XT 200 Å, 2.5 μm 4.6 x 150 mm, column at 40 °C, RI detection |
| Flow drift |
Flow precision over 24 hrs: <0.2% RSD for 24 injections (one per hour) based on 24 replicates of a polystyrene standard at 10 μL injection volume, 1.0 mL/min, 100% THF, ACQUITY APC XT 200 Å, 2.5 μm 4.6 x 150 mm, column at 40 °C, RI detection |
| Priming |
Automated priming at flow rate of 4 mL/min |
| Primary wetted materials |
302 SS, 316 SS, Nitronic 60, titanium alloy, fluoropolymer, UHMWPE blend, fluoroelastomer, PPS, zirconia, ruby, sapphire, DLC, gold, PEEK, MP35N, PEEK blend, polyimide |
| Polymer Quaternary Solvent Manager (p-QSM) |
| Number of solvents |
Blend up to four solvents in any miscible combination (standard)
Expanded solvent choices with optional six-port solvent select valve |
| Solvent degassing |
Integrated vacuum degassing, four chambers
One additional chamber for the SM-FTN purge solvent |
| Solvent blending |
Automated, on-line pH, ionic strength, and organic modifier blending from pure solvents with Auto•Blend™ Plus Technology |
| Gradient formation |
Low-pressure mixing, quaternary gradient |
| Gradient profiles |
11 gradient curves [including linear, step (2), concave (4), and convex (4)] |
| Primary check valve |
Intelligent Intake Valve (i² Valve) |
| Flow accuracy |
±1.0% at 0.5 to 2.0 mL/min using 100% A
Backpressure 1,000 psi ±200 psi with degassed H2O |
| Flow precision |
<0.075% RSD or 0.020 min SD, whichever is greater, based on six replicates 60:40 H2O/MeOH via premix, 0.5 mL/min, alkylphenone mix (5.0 μL injection volume), ACQUITY UPLC BEH C18, 1.7 μm, 2.1 x 50 mm, 35 °C ±0.1 °C, UV @ 254 nm |
| Composition ripple (baseline noise) |
<1.0 mAu (<0.1 mAU with optional 250.0 μL mixer) A: H2O + 0.1% TFA, B: ACN + 0.1% TFA, 0.5 mL/min, ACQUITY UPLC BEH C18, 1.7 μm, 2.1 x 50 mm UV @ 214 nm, 5 mm analytical flow cell |
| Composition accuracy |
±0.5% absolute (full scale) from 5% to 90% from 0.2 to 2.0 mL/min
Degassed ACN/ACN with caffeine at 12 mg/L concentration, back pressure 2000 psi, step gradient method, UV at 273 nm |
| Composition precision |
<0.15% RSD or ±0.04 min SD, whichever is greater, based on six replicate injections (with i² Valve)
60:40 H2O/MeOH via Auto•Blend Plus Technology, 0.5 mL/min, alkylphenone mix (5.0 μL 60:40 H2O/MeOH via Auto•Blend Plus Technology, 0.5 mL/min, alkylphenone mix (5.0 μL injection volume), ACQUITY UPLC BEH C18, 1.7 μm, 2.1 x 50 mm, 35 °C ± 0.1 °C, UV @ 254 nm |
| Compressibility compensation |
Automatic and continuous |
| Priming |
Wet priming can run at flow rates up to 4 mL/min |
| Pump seal wash |
Equipped with an automated wash system to flush the rear of the high-pressure seal and the plunger |
| Flow ramping |
Range: 0.01 to 30.00 min to reach 2.0 mL/min
Default: 0.45 min to reach 2.0 mL/min |
| Primary wetted materials |
316 SS, NITRONIC® 60, titanium alloy, fluoropolymer, UHMWPE blend, fluoroelastomer, PPS, zirconia, ruby, sapphire, DLC, gold, MP35N, PEEK blend, polyimide |
| Solvent selection |
Programable up to six solvents (optional) |
| Sample Manager With Flow-Through Needle |
| Injection volume range |
0.5 to 50.0 μL as standard
0.1 to 250.0 μL with optional extension loop |
| Injection needle wash |
Integral, active, programmable |
| Number of sample plates |
Two |
| Maximum sample capacity |
96 in 2 mL vial holders. For more information see Waters Sample Vials and Accessories brochure |
| Injection accuracy (aspiration) |
±0.2 μL, measured by water weight removed from vial with 10.0-μL injections over 20 injections using standard 100 μL syringe |
| Injection linearity |
>0.999 based from 0.5 μL to 50 μL injections of a polystyrene standard, 0.5 mL/min, 100% THF, ACQUITY APC XT 200 Å, 2.5 μm 4.6 x 150 mm, column at 40 °C, RI detection |
| Injection precision |
<0.5% RSD 2.0 to 50.0 μL based six replicates at each injection volume of a polystyrene standard, 0.5 mL/min, 100% THF, ACQUITY APC XT 200 Å, 2.5 μm 4.6 x 150 mm, column at 40 °C, RI detection |
| Sample compartment temperature range |
4.0 °C to 40.0 °C, settable in 0.1 °C increments with a tolerance range between -2 °C and +4 °C |
| Temperature accuracy |
±0.5 °C at the sample compartment temperature sensor |
| Temperature stability |
±1.0 °C at the sample compartment temperature sensor |
| Sample carryover |
<0.005% caffeine (UV) based on carryover after 4 mg/mL standard, 5 μL injection volume, 90/10 water/acetonitrile (for eluent, wash solvents, and diluent/blank), 0.6 mL/min, ACQUITY UPLC BEH C18 1.7 μm 2.1 x 50 mm, column at 40°C, UV detection at 273 nm |
| Primary wetted materials |
316 SS, PPS, polyimide, fluoropolymer |
| Single Zone Column Manager |
| Column capacity |
One bank of columns connected in series:
- Maximum of four 30 to 75 mm columns
- Maximum of three 150 mm columns
Two modules configurable per APC System |
| Valves |
Optional valves for column switching, column bypass, waste, or solvent recycling |
| Solvent conditioning |
Active pre-heating as standard |
| Column tracking |
Individual eCord™ connectors for all columns in the bank |
| Temperature range |
Controlled 4.0 °C to 90.0 °C settable in 0.1 °C increments |
| Time to temperature ready |
12 min after door open 30 s, at internal temperature sensor after 1-hour thermal equilibrium at set point |
| Temperature accuracy |
±0.5 °C measured at sensor on trough |
| Temperature precision |
±0.1 °C measured at sensor on trough |
| 30 cm Single Zone Column Manager (Optional) |
| Column capacity |
Support for one or two banks of up to four columns per bank:
- Maximum column length 300 mm, plus 50 mm guard column
- Maximum column I.D. 8 mm |
| Solvent conditioning |
Passive pre-heating (standard) |
| Column tracking |
Optional eCord connectors with support for up to four columns per bank |
| Temperature range |
Settable 20 °C to 90 °C in 0.1 °C increments
Controllable (ambient +5 °C) to 90 °C |
| Time to temperature ready |
12 min after door open 30 s, at internal temperature sensor after 1-hour thermal equilibration at 60 °C temperature set point, 25 °C ambient temperature. |
| Temperature accuracy |
±0.5 °C measured at sensor |
| Temperature precision |
±0.1 °C measured at sensor |
| Temperature stability |
±0.3 °C measured at sensor |
| Primary wetted materials (including optional valves) |
316 SS, DLC |
| p-QSM Instrument Control |
| Number of solvents |
Blend up to four solvents in any miscible combination (standard)
Expanded solvent choices with optional six-port solvent select valve |
| Solvent degassing |
Integrated vacuum degassing, four chambers
One additional chamber for the SM-FTN purge solvent |
| Solvent blending |
Automated, on-line pH, ionic strength, and organic modifier blending from pure solvents with Auto•Blend™ Plus Technology |
| Gradient formation |
Low-pressure mixing, quaternary gradient |
| Gradient profiles |
11 gradient curves [including linear, step (2), concave (4), and convex (4)] |
| Primary check valve |
Intelligent Intake Valve (i² Valve) |
| Flow accuracy |
±1.0% at 0.5 to 2.0 mL/min using 100% A
Backpressure 1,000 psi ±200 psi with degassed H2O |
| Flow precision |
<0.075% RSD or 0.020 min SD, whichever is greater, based on six replicates 60:40 H2O/MeOH via premix, 0.5 mL/min, alkylphenone mix (5.0 μL injection volume), ACQUITY UPLC BEH C18, 1.7 μm, 2.1 x 50 mm, 35 °C ±0.1 °C, UV @ 254 nm |
| Composition ripple (baseline noise) |
<1.0 mAu (<0.1 mAU with optional 250.0 μL mixer) A: H2O + 0.1% TFA, B: ACN + 0.1% TFA, 0.5 mL/min, ACQUITY UPLC BEH C18, 1.7 μm, 2.1 x 50 mm UV @ 214 nm, 5 mm analytical flow cell |
| Composition accuracy |
±0.5% absolute (full scale) from 5% to 90% from 0.2 to 2.0 mL/min
Degassed ACN/ACN with caffeine at 12 mg/L concentration, back pressure 2000 psi, step gradient method, UV at 273 nm |
| Composition precision |
<0.15% RSD or ±0.04 min SD, whichever is greater, based on six replicate injections (with i² Valve)
60:40 H2O/MeOH via Auto•Blend Plus Technology, 0.5 mL/min, alkylphenone mix (5.0 μL 60:40 H2O/MeOH via Auto•Blend Plus Technology, 0.5 mL/min, alkylphenone mix (5.0 μL injection volume), ACQUITY UPLC BEH C18, 1.7 μm, 2.1 x 50 mm, 35 °C ± 0.1 °C, UV @ 254 nm |
| Compressibility compensation |
Automatic and continuous |
| Priming |
Wet priming can run at flow rates up to 4 mL/min |
| Pump seal wash |
Equipped with an automated wash system to flush the rear of the high-pressure seal and the plunger |
| Flow ramping |
Range: 0.01 to 30.00 min to reach 2.0 mL/min
Default: 0.45 min to reach 2.0 mL/min |
| Primary wetted materials |
316 SS, NITRONIC® 60, titanium alloy, fluoropolymer, UHMWPE blend, fluoroelastomer, PPS, zirconia, ruby, sapphire, DLC, gold, MP35N, PEEK blend, polyimide |
| Solvent selection |
Programable up to six solvents (optional) |
| Physical / Environmental Specifications* |
| Acoustic noise |
<62 dBA, system |
| Operating temperature range |
15 °C to 40 °C (59 °F to 104 °F) |
| Operating humidity range |
20% to 80%, non-condensing |
| Dimensions |
Width: 34.3 cm (13.5 in.)
Height: 70.6 cm (27.8 in.)
Depth: 71.2 cm (28.0 in.) |
| Weight |
74.9 kg (165.0 lbs.) |
| *Core system with single CM-S only. |
| Electrical Specifications |
| Power requirements |
100–240 VAC |
| Line frequency |
50 Hz–60 Hz |
| Power consumption* |
1,000 VA |
| *Core system with single CM-S only. |
