RapiGest™ SF Surfactant is a reagent used to enhance enzymatic digestion of proteins. RapiGest SF helps solubilize proteins, making them more susceptible to enzymatic cleavage without inhibiting enzyme activity.
Unlike other commonly used denaturants, such as SDS or urea, RapiGest SF does not modify peptides or suppress protease activity. It is compatible with enzymes such as Trypsin, Lys-C, Asp-N and Glu-C and other enzymes.
- Improves efficiency of digestion
- No adducts or protein modifications observed
- Degraded at low pH and removed from the sample
- Compatible with HPLC and MS analysis
Waters reagent, RapiGest™ SF, drastically improves in-solution protein digestion in terms of speed and peptide recovery. RapiGest™ SF is available in 1mg vials containing enough material for 1-10 digestions. Add RapiGest™ SF to your proteolytic digestion, (using trypsin or other enzymes) to enhance digestion speed and increase peptide recovery.
Specifications
Applications | Life Science |
Detector | Mass Spec |
Product Type | Standard |
Shipping Formulation | Solid |
Storage | Frozen -20 C |
System | LC/MS |
- Model: 186002122
- Manufactured by: Waters